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KMID : 0545119970070010062
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Journal of Microbiology and Biotechnology
1997 Volume.7 No. 1 p.62 ~ p.67
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Characterization of Ethanol Fermentation Using Alginate Immobilized Thermotolerant Yeast Cells
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Sohn, Ho Yong
Park, Wan/Jin, Ingnyol/Seu Jung Hwn
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Abstract
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To enhance the hyperproductive and low energy-consuming ethanol fermentation rate, the thermotolerant yeast S. cerevisiae RA-74-2 cells were immobilized. An efficient immobilization condition was proved to be 1.5% (w/v) alginate solution, neutral pH and 20 h activation of beads. The fermentation characteristics and stability at various temperatures were examined as compared with free S. cerevisiae RA-74-2 cells. The immobilized cells had excellent fermentation rate at the range of pH 3∼7 at 30∼42℃ in 15∼20% glucose media. When the seed volume was adjusted to 0.12 (v/v) (6 ㎖ bead/50 ㎖ medium), 11% (w/v) ethanol was produced during the first 34 h and 12.15% (w/v) ethanol [95% (w/v) of theoretical yield] during the first 60 h in 25% glucose medium. In repetitive fermentation using a 2 litre fermentor, 5.79-7.27% (w/v) ethanol [76-95% (w/v) of theoretical yield] was produced during the 40-55 h in 15% glucose media. These data suggested the fact that alginate beads of thermotolerant S. cerevisiae RA-74-2 cells would contribute to economic and hyperproductive ethanol fermentation at high temperature.
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